■ 基本信息
別名: | Plasmid #79873;pAX01-dCas9 |
原核抗性: | Amp |
真核抗性: | Ery |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 枯草桿菌 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 枯草桿菌 |
質(zhì)粒用途: | 基因編輯 |
片段類型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
pJMP1質(zhì)粒是一個(gè)枯草桿菌基因編輯質(zhì)粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. ?PubMed??Journal
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK2006 pJMP1枯草編輯質(zhì)粒.txt
