■ 基本信息
別名: | 四環(huán)素激活 |
啟動子: | Tet |
復制子: | pUC |
質粒分類: | 慢病毒表達質粒 |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
5'測序引物: | 根據(jù)全序列設計 |
3'測序引物: | 根據(jù)全序列設計 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 蛋白表達 |
片段類型: | ORF |
片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標記: |
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■ 質粒簡介
慢病毒載體pLVX-TetOne-Puro是集調控與應答功能于一體的四環(huán)素誘導載體。The Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing mammalian cells. The all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. In addition, this vector contains a puromycin antibiotic selection marker.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1692 pLVX-TetOne-Puro慢病毒四環(huán)素激活表達質粒.txt
