■ 基本信息
啟動子: | PnisA |
復制子: | repA，repC |
終止子: | T |
質粒分類: | 乳酸乳球菌表達載體 |
質粒大小: | 3167bp |
原核抗性: | Chl |
真核抗性: | Chl |
克隆菌株: | MC1061 |
培養(yǎng)條件: | 30度 |
表達宿主: | NZ9000乳酸乳球菌 |
培養(yǎng)條件: | M17+0.5%葡萄糖/30度密封靜置液體培養(yǎng) |
5'測序引物: | 根據(jù)序列設計 |
■ 質粒屬性
質粒宿主: | 乳酸菌 |
質粒用途: | 蛋白表達 |
片段類型: |
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片段物種: |
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原核抗性: | Chl
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真核抗性: |
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熒光標記: |
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■ 質粒簡介
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII. The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus. pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1060pNZ8148乳酸菌分泌質粒.txt
質粒只保證關鍵序列正確，不保證表達效果。
