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產(chǎn)品名稱:pIEFBPR質(zhì)粒

貨號 規(guī)格 價格 訂購數(shù)量 是否現(xiàn)貨
ZK1030 1μg(20μl,50ng/μl) 1170 - + 有貨

基本信息

復制子:

ColE1 Ori

質(zhì)粒分類:

枯草桿菌質(zhì)粒;枯草整合質(zhì)粒;枯草分泌質(zhì)粒

質(zhì)粒大小:

8965bp

原核抗性:

Amp

克隆菌株:

DH5a

培養(yǎng)條件:

37度


質(zhì)粒屬性

質(zhì)粒宿主:

枯草桿菌

質(zhì)粒用途:

蛋白表達

片段類型:

ORF

片段物種:


原核抗性:

Amp

真核抗性:


熒光標記:



質(zhì)粒簡介

pIEFBPR質(zhì)粒是一個枯草整合型分泌載體,該質(zhì)粒的參考文獻:mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis

ColE1 ori, ColE1 replication origin; amp, ampicillin-resistance marker; spc, spectinomycin-resistance marker; lacI, lac-repressor-encoding gene; Pspac, Pspac promoter; mazF, E.coli toxin gene; Plpp, promoter of E.coli lpp gene; mazE, E.coli antitoxin gene; DR, directly-repeated sequence; amyE-front and amyE-back, upstream and downstream sequences of the B.subtilis amyE gene, respectively; bpr-front and bpr-back, upstream and downstream sequences of the B.subtilis bpr gene, respectively. All of the DNA sequences located between the amyE-front and amyE-back or the bpr-front and bpr-back allow integration into the chromosome of B.subtilis via a double-crossover event, resulting in selection for spectinomycin-resistance. The unique restriction enzyme sites are highlighted in blue.


質(zhì)粒圖譜


質(zhì)粒序列

質(zhì)粒序列請下載:ZK1030pIEFBPR枯草分泌質(zhì)粒.txt


質(zhì)粒只保證關鍵序列正確,不保證表達效果。

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總價格:¥2000