■ 基本信息

■ 質粒屬性

■ 質粒簡介

All vectors use the strong promoter preceding the groESL operon of Bacillus subtilis fused to the lac operator allowing their induction by addition of IPTG. While the background level of expression of these expression cassettes is very low in the absence of the inducer, an induction factor of about 1,300 was measured using the bgaB reporter gene. The amount of recombinant protein produced after addition of IPTG may represent 10 and 13%, respectively, of the total cellular protein. High level secretion of amyQ α-amylase and cellulase A and B of Clostridium thermocellum was demonstrated. An efficient Shine-Dalgarno sequence as well as a multiple cloning site (BamH I, Xba I, AatII, SmaI) were also inserted. To obtain secretion of recombinant proteins, the coding region for the signal peptide of the amyQ gene encoding an α-amylase was fused to the SD sequence of pHT01, thereby constructing pHT43.

大腸桿菌-枯草芽孢桿菌穿梭質粒的表達載體pHT01可以在枯草芽孢桿菌中高效表達重組外源蛋白。載體基于強σA-依賴性啟動子的枯草桿菌groE操縱子，通過添加lac操縱子改造成為一種高效可控的（IPTG誘導的）啟動子。

■ 質粒圖譜

■ 質粒序列

質粒序列請下載：ZK1011pHT01枯草胞內質粒.txt

質粒只保證關鍵序列正確，不保證表達效果。