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莊盟生物

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產品名稱:pGBT9

貨號 規(guī)格 價格 訂購數(shù)量 是否現(xiàn)貨
ZK983 1μg(20μl,50ng/μl) 870 - + 有貨

基本信息

啟動子:

ADH1

復制子:

質粒分類:

酵母系列,酵母雙雜交載體

質粒大小:

5524bp

原核抗性:

Amp

真核抗性:

TRP1

克隆菌株:

DH5a

培養(yǎng)條件:

37度

表達宿主:

酵母細胞

5'測序引物:

根據序列設計引物

3'測序引物:

根據序列設計引物


質粒屬性

質粒宿主:

酵母菌

質粒用途:

雜交

片段類型:


片段物種:


原核抗性:

Amp

真核抗性:

TRP1

熒光標記:



質粒簡介

pGBT9 generates a hybrid protein that contains the sequences for the GAL4 DNA-binding domain (DNA-BD; a.a. 1–147). For the construction of a hybrid protein, the gene encoding the protein of interest is ligated into the MCS in the correct orientation and in the correct reading frame such that a fusion protein is generated. The fusion protein is expressed in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that are an intrinsic part of the GAL4 DNA-BD . pGBT9 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae. It carries the bla gene (for ampicillin resistance in E. coli) and the TRP1 nutritional marker that allow yeast auxotrophs carrying pGBT9 to grow on limiting synthetic medium lacking Trp.


質粒圖譜


質粒序列

質粒序列請下載:ZK983pGBT9酵母雙雜交質粒.txt

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總價格:¥2000