■ 基本信息
啟動(dòng)子: | TRE |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal，beta-globin polyA signal |
質(zhì)粒分類: | 哺乳細(xì)胞，四環(huán)素調(diào)控系統(tǒng)載體 |
質(zhì)粒大小: | 5108bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 四環(huán)素誘導(dǎo) |
5'測(cè)序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: |
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pTRE2pur is a response plasmid that expresses a gene of interest (Gene X) in Clontech's Tet-On? and Tet-Off? Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On).pTRE2pur contains an MCS immediately downstream of the Tet-responsive PhCMV*-1 promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. PhCMV*-1 contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). TheTRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, PhCMV*-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. Note that the cloned insert must have an initiation codon. In some cases the addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. The puromycin resistance gene is used to directly select for stable transformants.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK692pTRE2pur哺乳調(diào)控質(zhì)粒.txt
