■ 基本信息
別名: | pET3a |
啟動(dòng)子: | T7 |
復(fù)制子: | pBR322 |
終止子: | T7 terminator |
質(zhì)粒大小: | 4640bp |
質(zhì)粒標(biāo)簽: | N-T7 |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37℃，有氧，LB |
表達(dá)宿主: | 大腸桿菌BL21(DE3) |
誘導(dǎo)方式: | 無需誘導(dǎo) |
5'測(cè)序引物: | T7:TAATACGACTCACTATAGGG |
3'測(cè)序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
備注: | 組成型表達(dá) |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 大腸桿菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡(jiǎn)介
pET-3a載體是一個(gè)大腸桿菌表達(dá)載體，T7啟動(dòng)子驅(qū)動(dòng)T7標(biāo)簽和目的基因融合表達(dá)。該載體是組成型表達(dá)，并且不攜帶6×HIS標(biāo)簽。The pET-3a-d vectors carry an N-terminal T7?Tag sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-23a-d(+) series corresponds to pET-3a-d but incorporates several additional features. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. 質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK141pET-3a大腸表達(dá)質(zhì)粒.txt
